Extended depth-of-field microscopy with a high-speed deformable mirror
نویسندگان
چکیده
منابع مشابه
Novel Deconvolution Kernel for Extended Depth-of-Field Microscopy with a High-Speed Deformable Mirror
A deformable mirror is used to scan the focal plane during the camera exposure, obtaining extended depth-of-field. A deconvolution kernel is approximated for a given scan depth and used to de-blur the image. OCIS codes: (180.2520) Fluorescence microscopy; (110.1080) Active or adaptive optics; (100.1830) Deconvolution
متن کاملExtended depth-of-field microscopy with a high-speed deformable mirror.
We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are...
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ژورنال
عنوان ژورنال: Optics Letters
سال: 2017
ISSN: 0146-9592,1539-4794
DOI: 10.1364/ol.42.000995